Simonati A, Cavanagh J B
Muscle Nerve. 1984 Jun;7(5):355-61. doi: 10.1002/mus.880070503.
Qualitative and quantitative morphological studies of the sternocostalis muscle innervation were made on rats chronically intoxicated with 2,5 hexanedione (2,5 HD) using the zinc iodide-osmium (ZIO) technique. Two distinct phases were seen in the events at the motor endplate. First, the number of motor endplates forming spontaneous terminal sprouts was found to increase linearly with time and, from the third week onward, the sprouts appeared to become progressively elongated. This latter change was associated with the appearance of swollen axons within intramuscular nerve bundles. Second, from the sixth week onward, wallerian degeneration of nerve fibers was seen and terminal sprouts began to make new arborizations on muscle fibers. By the eighth week, this occurred in as many as 66% of the rats, and collateral sprouting was also observed at this time. The occurrence of increased spontaneous terminal sprouting due to altered neuromuscular function is discussed in the light of axonal changes resulting from neurofilament accumulation following 2,5 HD intoxication.
采用碘化锌锇（ZIO）技术，对长期用2,5 -己二酮（2,5 HD）中毒的大鼠胸肋肌神经支配进行了定性和定量形态学研究。在运动终板处观察到两个不同阶段。首先，发现形成自发终末芽的运动终板数量随时间呈线性增加，从第三周起，芽似乎逐渐伸长。后一种变化与肌内神经束内轴突肿胀的出现有关。其次，从第六周起，可见神经纤维的华勒氏变性，终末芽开始在肌纤维上形成新的分支。到第八周，多达66%的大鼠出现这种情况，此时还观察到侧支发芽。根据2,5 HD中毒后神经丝积累导致的轴突变化，讨论了由于神经肌肉功能改变导致自发终末芽增加的发生情况。
