Shafiq S A, Shimizu T, Fischman D A
Muscle Nerve. 1984 Jun;7(5):380-7. doi: 10.1002/mus.880070507.
Monoclonal antibodies (McAbs) were generated against slow myosin from the chicken anterior latissimus dorsi (ALD) muscle and their reactivity was checked against fast (pectoralis), slow (ALD), cardiac (ventricular), and smooth (gizzard) myosins by radioimmunoassay (RIA), immunoautoradiography (immunoblots), and indirect immunofluorescence techniques. In RIAs, the McAb (ALD-58) described in this article reacted specifically with slow myosin, with only a weak cross-reactivity to cardiac myosin. In immunoblots against whole muscle homogenates and purified myosins, it bound selectively to the 200 Kd myosin heavy chain band. The ALD-58 antibody stained the fibers of the ALD muscle uniformly but gave three grades of reactions (strong, weak, and negative), with histochemically identified type 1 fibers of sartorius and gastrocnemius muscles demonstrating the immunological heterogeneity of myosins in type 1 skeletal muscle fibers.
制备了针对鸡背阔肌（ALD）慢肌球蛋白的单克隆抗体（McAbs），并通过放射免疫分析（RIA）、免疫放射自显影（免疫印迹）和间接免疫荧光技术检测其对快肌（胸肌）、慢肌（ALD）、心肌（心室肌）和平滑肌（砂囊肌）肌球蛋白的反应性。在RIA中，本文所述的单克隆抗体（ALD-58）与慢肌球蛋白特异性反应，与心肌球蛋白仅有微弱的交叉反应。在针对全肌肉匀浆和纯化肌球蛋白的免疫印迹中，它选择性地结合到200 Kd肌球蛋白重链条带。ALD-58抗体均匀地染色ALD肌肉的纤维，但产生三种反应等级（强、弱和阴性），缝匠肌和腓肠肌经组织化学鉴定的1型纤维显示出1型骨骼肌纤维中肌球蛋白的免疫异质性。
