Weis R M, McConnell H M
Nature. 1984;310(5972):47-9. doi: 10.1038/310047a0.
Epifluorescence optical microscopy has been used to show the formation of solid phase domains from fluid phase domains on compression of dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface. These monolayers can be transferred to solid substrates, and in certain conditions exhibit X-ray diffraction characteristic of highly ordered two-dimensional crystals. In previous work, the crystal domains, visualized by the exclusion of fluorescent lipid probes, were often round and arrayed within a continuous fluid phase domain in a hexagonal pattern. We report here that when DPPC monolayers are more rapidly compressed, at a rate of the order of a 2% decrease in area per second, chiral solid domains of lipid are formed. The handedness of the solid domains is directly related to the enantiomorphic configuration of the lipids composing the monolayer. The shape of these domains provides direct visual evidence for long range orientational order in two-dimensional crystals.
落射荧光光学显微镜已被用于展示在气-水界面压缩二棕榈酰磷脂酰胆碱（DPPC）单层时，流体相区域形成固相区域的过程。这些单层可以转移到固体基质上，并且在某些条件下呈现出高度有序的二维晶体的X射线衍射特征。在之前的工作中，通过排除荧光脂质探针可视化的晶体区域通常是圆形的，并以六边形图案排列在连续的流体相区域内。我们在此报告，当以每秒约2%的面积减少率更快地压缩DPPC单层时，会形成脂质的手性固相区域。固相区域的手性与构成单层的脂质的对映体构型直接相关。这些区域的形状为二维晶体中的长程取向有序提供了直接的视觉证据。
