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The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation.

作者信息

Wisher M H, Rose M E

出版信息

Parasitology. 1984 Jun;88 ( Pt 3):515-9. doi: 10.1017/s0031182000054779.

DOI:10.1017/s0031182000054779
PMID:6739136
Abstract

A method is described for the reproducible purification of greater than 1.0 X 10(8) sporozoites of Eimeria spp. by centrifugation on metrizamide density-gradients. The mean contamination of the purified sporozoite fraction by sporocysts and sporocyst debris was less than 6% and the recovered sporozoites were fully viable, infective and ultrastructurally intact.

摘要

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