Rolin O, Zerial A, Bouanchaud D H
Pathol Biol (Paris). 1984 May;32(5):318-21.
When comparing antibiotic activities, it might be of interest to study parameters other than minimal inhibitory concentrations (MIC's). Specific activity of beta-lactams on bacterial cell wall makes it possible to determine radiolabelled diaminopimelic acid (DAP) incorporation in growing cultures. We have studied the effects of various concentrations of cefalotin , cefotaxime, latamoxef (moxalactam) and ceftiolene (42 980 RP) on DAP incorporation in 6 strains of E. coli, E. cloacae and S. aureus. Drug concentration which inhibits 90 % of radioactivity incorporation (CII 90) was found to vary from 0.1 X MIC to 3 X MIC. This fact suggests that beta-lactam action on cell wall synthesis and/or structure and MIC's are not always strictly correlated.
在比较抗生素活性时，研究最低抑菌浓度（MIC）以外的参数可能会很有意思。β-内酰胺类药物对细菌细胞壁的特异性作用使得在生长培养物中测定放射性标记的二氨基庚二酸（DAP）掺入量成为可能。我们研究了不同浓度的头孢噻吩、头孢噻肟、拉氧头孢（羟羧氧酰胺菌素）和头孢硫脒（42 980 RP）对6株大肠杆菌、阴沟肠杆菌和金黄色葡萄球菌中DAP掺入的影响。发现抑制90%放射性掺入的药物浓度（CII 90）在0.1×MIC到3×MIC之间变化。这一事实表明，β-内酰胺类药物对细胞壁合成和/或结构的作用与MIC并不总是严格相关的。
