Blalock T L, Thaxton J P
Poult Sci. 1984 Jun;63(6):1243-9. doi: 10.3382/ps.0631243.
An economical vitamin B6-deficient ration, which was palatable to broiler chickens, was prepared and fed to 1-day-old broiler chicks. The experimental ration was a glucose-soybean meal diet. Vitamin B6 was removed by washing the soybean meal with water. Microbiological analysis revealed that the washed ration contained .45 mg vitamin B6 activity/kg. Experimental rations were formulated to contain .5, 1.0, and 3.0 mg added pyridoxine HCl/kg of ration. These supplemental levels produced total pyridoxine concentrations, as assayed, of .95, 1.48, and 3.18 mg pyridoxine HCl activity/kg of diet. Chicks were grown to 7 weeks of age and characteristic B6 deficiency traits, including increased mortality, decreased body weight gain, and increased incidence of abnormal leg conformation, were quantitated or observed. An extensive hematological evaluation at 4 weeks of age indicated that this marginal B6 deficiency resulted in increased erythrocyte numbers, decreased mean corpuscular hemoglobin levels, and increased erythrocyte fragility. No changes in hematocrits, total hemoglobin level, intracellular hemoglobin concentration, or reticulocyte number were found. These results indicate that anemia did not occur in broilers experiencing a moderate vitamin B6 deficiency. The hematological condition is described as microcytic, normochromic polycythemia.
制备了一种经济实惠且肉仔鸡适口的维生素B6缺乏日粮，并饲喂1日龄的肉仔鸡。实验日粮为葡萄糖-豆粕日粮。通过用水冲洗豆粕来去除维生素B6。微生物分析表明，冲洗后的日粮每千克含有0.45毫克维生素B6活性。将实验日粮配制成每千克日粮添加0.5、1.0和3.0毫克盐酸吡哆醇。这些补充水平经测定后产生的总吡哆醇浓度分别为每千克日粮0.95、1.48和3.18毫克盐酸吡哆醇活性。将肉仔鸡饲养至7周龄，对包括死亡率增加、体重增加减少和腿部异常形态发生率增加等典型的维生素B6缺乏特征进行了定量或观察。在4周龄时进行的广泛血液学评估表明，这种边缘性维生素B6缺乏导致红细胞数量增加、平均红细胞血红蛋白水平降低和红细胞脆性增加。未发现血细胞比容、总血红蛋白水平、细胞内血红蛋白浓度或网织红细胞数量有变化。这些结果表明，中度维生素B6缺乏的肉仔鸡未发生贫血。血液学状况被描述为小细胞、正常色素性红细胞增多症。
