Pintar J E, Schachter B S, Herman A B, Durgerian S, Krieger D T
Science. 1984 Aug 10;225(4662):632-4. doi: 10.1126/science.6740329.
Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.
对从成年大鼠睾丸中分离出的总RNA和聚腺苷酸化RNA进行的Northern印迹分析表明，这些提取物中存在一种促阿片-黑素细胞皮质素原（POMC）样信使RNA分子。睾丸POMC信使RNA的长度与杏仁核和中脑POMC信使RNA相当，并且似乎比在下丘脑以及垂体前叶和中叶中发现的POMC信使RNA至少短200个核苷酸。原位杂交显示，POMC信使RNA位于睾丸间质细胞中，而睾丸间质细胞是睾丸中唯一含有可免疫染色的POMC衍生肽的细胞。这些结果表明，POMC在睾丸中进行局部合成。
