Takeda H, Yano T, Kishikawa H, Kobori S, Uzawa H
Thromb Res. 1984 May 15;34(4):321-31. doi: 10.1016/0049-3848(84)90389-x.
Platelet phospholipase plays an important role in the metabolic responses of platelets to exogenous stimuli. The platelet phospholipase activity (PLA) was therefore studied in 38 patients with ischemic heart disease (IHD) and in 26 age-matched normal subjects who served as controls. The mean platelet PLA in the IHD group was 12.72 +/- 1.03 nmol/mg protein/30 sec which was significantly (p less than 0.005) higher than that of the normal controls (8.72 +/- 0.76). When they were classified into acute stage, such as unstable angina or acute myocardial infarction (AMI), and chronic stage, such as stable angina or old myocardial infarction (OMI), there was no significant difference between them. On the other hand, about two-fold activation of platelet PLA was observed in acute stage IHD, and 20-30% inhibition of it was demonstrated in chronic stage IHD following the addition of autologous plasma to washed platelet suspensions, suggesting that certain plasma factor(s) are responsible for such phenomena. In an attempt to identify these plasma factor(s), various substances such as serum albumin, high density lipoprotein, prostaglandin E1 (PGE1) and E2 (PGE2), and platelet activating factor were assessed by in vitro experiments. Only PGE1 and PGE2 revealed a significant effect on the platelet PLA. The relationship between plasma and platelet activity in terms of platelet PLA deserves attention since it varies according to the type and stage of IHD.
血小板磷脂酶在血小板对外源性刺激的代谢反应中起重要作用。因此，我们对38例缺血性心脏病（IHD）患者和26例年龄匹配的正常对照者的血小板磷脂酶活性（PLA）进行了研究。IHD组的平均血小板PLA为12.72±1.03 nmol/mg蛋白/30秒，显著高于正常对照组（8.72±0.76）（p<0.005）。将患者分为急性期，如不稳定型心绞痛或急性心肌梗死（AMI），以及慢性期，如稳定型心绞痛或陈旧性心肌梗死（OMI），两组之间无显著差异。另一方面，在急性期IHD中观察到血小板PLA约有两倍的激活，而在慢性期IHD中，向洗涤后的血小板悬液中加入自体血浆后，血小板PLA受到20%-30%的抑制，这表明某些血浆因子导致了这种现象。为了鉴定这些血浆因子，通过体外实验评估了各种物质，如血清白蛋白、高密度脂蛋白、前列腺素E1（PGE1）和E2（PGE2）以及血小板活化因子。只有PGE1和PGE2对血小板PLA有显著影响。鉴于IHD的类型和阶段不同，血浆与血小板PLA活性之间的关系值得关注。
