Neis J M, Van Gemert P J, Roelofs H M, Henderson P T
Toxicology. 1984 Jun;31(3-4):319-27. doi: 10.1016/0300-483x(84)90113-6.
The effect of alkylating agents on the content of free SH-groups in human, rat and rabbit hemoglobin was examined. Treatment of blood in vitro with iodoacetamide, styrene oxide and methyl methanesulfonate resulted in a dose-dependent decrease of free SH-groups in hemoglobin. The free SH-groups of rat hemoglobin were more susceptible to the alkylating agents tested than those of human and rabbit hemoglobin. The method is based on the isolation of hemoglobin and a subsequent measurement of the free SH-content of the hemoglobin by means of a spectrophotometric procedure. This procedure may be useful to examine differences in susceptibility of free SH-groups in hemoglobin, e.g. of different animal species, and on the other hand, to study the potencies of different electrophilic agents to alkylate these nucleophilic centers.
研究了烷化剂对人、大鼠和兔血红蛋白中游离巯基含量的影响。用碘乙酰胺、环氧苯乙烷和甲磺酸甲酯体外处理血液，导致血红蛋白中游离巯基呈剂量依赖性减少。大鼠血红蛋白的游离巯基比人和兔血红蛋白的游离巯基对所测试的烷化剂更敏感。该方法基于血红蛋白的分离以及随后通过分光光度法测量血红蛋白的游离巯基含量。该方法可能有助于检测血红蛋白中游离巯基（如不同动物物种的游离巯基）敏感性的差异，另一方面，有助于研究不同亲电试剂烷基化这些亲核中心的能力。
