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[同时测定细菌脲酶和苯丙氨酸脱氨酶的快速方法]

[Rapid method for the simultaneous determination of bacterial urease and phenylalanine desaminase].

作者信息

Pavlov A, Markarian M

出版信息

Vet Med Nauki. 1984;21(3):58-61.

PMID:6740921
Abstract

Tested was an accelerated method for the evaluation of urease and phenylalanine desaminase of a total of 327 strains of E. coli, S. typhimurium, S. enteritidis, S. braenderup, S. tennessee, P. vulgaris, P. mirabilis, Yersinia enterocolitica, and Citrobacter that were subjected to a preliminary identification with routine methods. It was established that the method tested had the following advantages as compared to the classical methods employed up to then in the laboratory practice: --100% positive results were obtained up to the 4th hour, i. e. the method appeared 6 times more rapid, and the overall result was obtained one day earlier; --the two enzymes were studied simultaneously; --the expenditures in connection with the use of nutrient medium per one test were several times lower. Suggested is the timely adoption of the accelerated method in the laboratory practice.

摘要

对总共327株大肠杆菌、鼠伤寒沙门氏菌、肠炎沙门氏菌、布伦德里普沙门氏菌、田纳西沙门氏菌、普通变形杆菌、奇异变形杆菌、小肠结肠炎耶尔森氏菌和柠檬酸杆菌进行了脲酶和苯丙氨酸脱氨酶评估的加速方法测试,这些菌株已用常规方法进行了初步鉴定。结果表明,与实验室实践中当时使用的经典方法相比,所测试的方法具有以下优点:——到第4小时获得了100%的阳性结果,即该方法快6倍,且总体结果提前一天获得;——同时研究了两种酶;——每次测试使用营养培养基的费用降低了几倍。建议在实验室实践中及时采用该加速方法。

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