Fried B, Pallone A
Z Parasitenkd. 1984;70(3):297-302. doi: 10.1007/BF00927815.
Various factors that influence in vitro pairing of Echinostoma revolutum adults were studied. More worms paired in Locke's, Ringer's, and 0.85% NaCl solutions than in the defined medium NCTC 135. Also, more pairing occurred at 39 or 43 degrees C than at 35 degrees C. Echinostomes placed 1, 3, or 5 cm apart in 5.5 cm diameter Petri dishes as well as those placed 2, 4, or 6 cm apart in 8.5 cm diameter Petri dishes paired; however, worms placed 8 cm apart in the latter dishes did not. Individuals maintained in Locke's solution at 20 or 39 degrees C for 1 h after having been removed from the chicks paired less frequently than those used for experiments immediately after their removal from chicks. Worms showed more pairing in fresh Locke's solution than in this solution preconditioned by excretory-secretory products of E. revolutum. Scanning electron microscopy revealed no differences between the tegument of fresh worms and of those maintained under experimental conditions for 2 h at 39 degrees C.
研究了影响卷棘口吸虫成虫体外配对的各种因素。与在限定培养基NCTC 135中相比，在洛克氏液、任氏液和0.85%氯化钠溶液中有更多的虫体配对。此外，在39或43℃时的配对比在35℃时更多。在直径5.5 cm的培养皿中相距1、3或5 cm放置的棘口吸虫以及在直径8.5 cm的培养皿中相距2、4或6 cm放置的棘口吸虫会配对；然而，在后者培养皿中相距8 cm放置的虫体则不会。从雏鸡体内取出后在20或39℃的洛克氏液中保存1小时的个体，其配对频率低于刚从雏鸡体内取出就用于实验的个体。与用卷棘口吸虫排泄-分泌产物预处理过的溶液相比，虫体在新鲜的洛克氏液中表现出更多的配对。扫描电子显微镜显示，新鲜虫体的体表与在39℃实验条件下保存2小时的虫体体表之间没有差异。
