Kanchurin A Kh, Petrova N S, Berzhets V M
Zh Mikrobiol Epidemiol Immunobiol. 1984 May(5):108-10.
The composition of cereal allergens was studied and the specific activity of the fractions isolated from these allergens was determined. Four protein fractions were obtained from wheat flour by the method of salting out. None of the fractions thus obtained exceeded the whole wheat allergen in specific activity as determined in the indirect mast-cell degranulation (IMCD) test. Three fractions were isolated by fractionation through Sephadex G-75. Of these, the first fraction possessed better physical properties, and its specific activity exceeded that of the initial allergen in the IMCD test.
对谷物过敏原的成分进行了研究,并测定了从这些过敏原中分离出的各组分的比活性。通过盐析法从小麦粉中获得了四种蛋白质组分。通过间接肥大细胞脱颗粒(IMCD)试验测定,所获得的这些组分中没有一个在比活性上超过全小麦过敏原。通过Sephadex G - 75分级分离得到了三个组分。其中,第一个组分具有更好的物理性质,并且在IMCD试验中其比活性超过了初始过敏原。