悬浮状态下鲎横纹肌经木瓜蛋白酶处理后的粗肌丝的动态激光光散射
Dynamic laser light scattering of papain-treated thick filaments from limulus striated muscle in suspension.
作者信息
Fan S F, Dewey M M, Colflesh D, Brink P, Chu B
出版信息
Adv Exp Med Biol. 1984;170:89-92. doi: 10.1007/978-1-4684-4703-3_8.
PMID:6741723
Abstract
Using quasielastic light scattering we have previously shown an increase in high frequency internal motion of isolated thick filament upon activation. This we have attributed to cross-bridge motion. Here we show that after cleavage of the S1 moiety of myosin from isolated filaments with papain, calcium ions no longer activate the isolated filaments to produce high-frequency motions.
摘要
我们先前利用准弹性光散射表明，激活后分离出的粗肌丝的高频内部运动增强。我们将此归因于横桥运动。在此我们表明，用木瓜蛋白酶从分离出的肌丝上切割掉肌球蛋白的S1部分后，钙离子不再激活分离出的肌丝以产生高频运动。
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