Backman L, Shanbhag V P
Anal Biochem. 1984 May 1;138(2):372-9. doi: 10.1016/0003-2697(84)90824-8.
Partition in aqueous two-phase systems should have great potentialities for studies of biological material. However, the general use of the method has probably been hindered by the difficulties in finding the composition of two-phase systems with desired qualities. Experimental design has proved to be of immense value for optimization in many different areas and should also be useful in selecting the best possible two-phase system. Therefore the simplex method has been applied to the search for the composition of an aqueous two-phase system in which the partition behavior of filamentous actin and human spectrin differ as much as possible.
双水相系统中的分配对于生物材料的研究应该具有很大的潜力。然而，该方法的普遍应用可能受到难以找到具有所需性质的双水相系统组成的阻碍。实验设计已被证明在许多不同领域的优化中具有巨大价值，在选择最佳的双水相系统方面也应该是有用的。因此，单纯形法已被应用于寻找一种双水相系统的组成，在该系统中丝状肌动蛋白和人血影蛋白的分配行为尽可能不同。
