Paye M, Beaune P, Kremers P, Guengerich F P, Letaw-Goujon F, Gielen J
Biochem Biophys Res Commun. 1984 Jul 18;122(1):137-42. doi: 10.1016/0006-291x(84)90450-9.
An enzyme linked immunosorbent assay (ELISA) using monoclonal and polyclonal antibodies has been developed to quantify individual cytochrome P-450 isoenzymes in microsomal preparations, namely UT-A and PB-B. This very sensitive method can be used for the rapid processing of large quantities of determinations and requires only limited amounts of antibodies.
已开发出一种使用单克隆抗体和多克隆抗体的酶联免疫吸附测定(ELISA)法,用于定量微粒体制剂中的个体细胞色素P - 450同工酶,即UT - A和PB - B。这种非常灵敏的方法可用于大量测定的快速处理,并且只需要有限量的抗体。