DNA向塑料膜的碱性转移

Alkaline transfer of DNA to plastic membrane.

作者信息

Chomczynski P, Qasba P K

出版信息

Biochem Biophys Res Commun. 1984 Jul 18;122(1):340-4. doi: 10.1016/0006-291x(84)90480-7.

Abstract

DNA forms a stable complex with Gene Screen Plus membrane at alkaline pH. Based on this, a method of alkaline transfer of DNA from agarose gel to Gene Screen Plus membrane was elaborated. The procedure entails the use of 0.4 M NaOH for both, the DNA denaturation and DNA transfer steps. The alkaline transfer offers a higher hybridization efficiency and simplifies the transfer procedure as compared with the standard method of DNA transfer at neutral pH. In addition, it can be used to remove RNA contamination from the transferred DNA.

摘要

在碱性pH条件下，DNA与基因筛选增强型膜形成稳定的复合物。基于此，阐述了一种将DNA从琼脂糖凝胶碱性转移至基因筛选增强型膜的方法。该程序在DNA变性和转移步骤中均使用0.4M氢氧化钠。与中性pH下的DNA转移标准方法相比，碱性转移具有更高的杂交效率并简化了转移程序。此外，它可用于去除转移DNA中的RNA污染。