Kemp P, Lander D J, Holman R T
Br J Nutr. 1984 Jul;52(1):171-7. doi: 10.1079/bjn19840084.
The hydrogenation of all the methylene-interrupted cis,cis-octadecadienoic acids was examined using pure cultures of six rumen bacteria able to hydrogenate linoleic acid to stearic acid or its immediate precursor, trans-11-octadecenoic acid, after first conjugating the linoleic acid to cis,trans-9,11-octadecadienoic acid. Only the delta 14-cis,17-cis-isomer was not hydrogenated by at least one of the bacteria and no evidence was found that conjugation was necessary before hydrogenation except for the delta 2-cis,5-cis- and delta 9-cis,12-cis-isomers. Several isomers were hydrogenated to an extent close to that achieved with linoleic acid (delta 9-cis,12-cis). Those bacteria only able to hydrogenate linoleic to trans-11-octadecenoic acid gave only octadecenoic acid products and those bacteria able to hydrogenate linoleic acid to stearic gave variable yields of octadecenoic acids and stearic acid except with the isomers delta 12-cis,15-cis and delta 13-cis,16-cis when only octadecenoic acids were detected. At the substrate levels used (20 micrograms/ml), both inhibition and stimulation of growth were found but no common pattern emerged, nor was the growth consistently related to the extent of hydrogenation.
使用六种瘤胃细菌的纯培养物对所有亚甲基间断的顺式、顺式-十八碳二烯酸的氢化作用进行了研究。这些细菌能够在将亚油酸共轭为顺式、反式-9,11-十八碳二烯酸后，将亚油酸氢化为硬脂酸或其直接前体反式-11-十八碳烯酸。只有δ14-顺式,17-顺式异构体未被至少一种细菌氢化，并且除了δ2-顺式,5-顺式和δ9-顺式,12-顺式异构体之外，没有发现氢化之前共轭是必需的证据。几种异构体的氢化程度接近于亚油酸（δ9-顺式,12-顺式）的氢化程度。那些只能将亚油酸氢化为反式-11-十八碳烯酸的细菌只产生十八碳烯酸产物，而那些能够将亚油酸氢化为硬脂酸的细菌除了在仅检测到十八碳烯酸的δ12-顺式,15-顺式和δ13-顺式,16-顺式异构体的情况下，产生的十八碳烯酸和硬脂酸产量各不相同。在所使用的底物水平（20微克/毫升）下，发现了生长抑制和生长刺激，但没有出现共同模式，生长也与氢化程度没有始终如一的关联。
