Karukstis K K, Sauer K
Biochim Biophys Acta. 1984 Jul 27;766(1):141-7. doi: 10.1016/0005-2728(84)90225-1.
The detailed process of excitation transfer among the antenna pigments of the red alga Porphyra perforata was investigated by measuring time-resolved fluorescence emission spectra using a single-photon timing system with picosecond resolution. The fluorescence decay kinetics of intact thalli at room temperature revealed wavelength-dependent multi-component chlorophyll a fluorescence emission. Our analysis attributes the majority of chlorophyll a fluorescence to excitation originating in the antennae of PS II reaction centers and emitted with maximum intensities at 680 and 740 nm. Each of these fluorescence bands was characterized by two kinetic decay components, with lifetimes of 340-380 and 1700-2000 ps and amplitudes varying with wavelength and the photochemical state of the PS II reaction centers. In addition, a small contribution to the long-wavelength fluorescence band is proposed to arise from chlorophyll a antennae coupled to PS I. This component displays fast decay kinetics with a lifetime of approx. 150 ps. Desiccation of the thalli dramatically increases the contribution of this fast decay component.
通过使用具有皮秒分辨率的单光子计时系统测量时间分辨荧光发射光谱，研究了红藻孔石莼天线色素之间激发转移的详细过程。室温下完整叶状体的荧光衰减动力学揭示了波长依赖性的多组分叶绿素a荧光发射。我们的分析将大部分叶绿素a荧光归因于源自PS II反应中心天线的激发，并在680和740 nm处发射出最大强度。这些荧光带中的每一个都由两个动力学衰减组分表征，寿命分别为340 - 380和1700 - 2000 ps，其幅度随波长和PS II反应中心的光化学状态而变化。此外，有人提出长波长荧光带的一小部分贡献来自与PS I耦合的叶绿素a天线。该组分显示出快速衰减动力学，寿命约为150 ps。叶状体的干燥显著增加了这种快速衰减组分的贡献。
