Stolz A, Yamada T, Sugiyama Y, Belknap W, Kaplowitz N
Biochim Biophys Acta. 1984 Jul 30;800(2):171-7. doi: 10.1016/0304-4165(84)90057-6.
We have recently identified a new group of proteins in rat liver cytosol which possess binding properties comparable to those of the glutathione-S-transferases, yet lack transferase activity. The bulk of organic anion binding in this fraction was associated with the tetrameric protein, designated the organic anion binder, molecular weight 34 000 (Sugiyama, Y., Yamada, T. and Kaplowitz, N. (1982) Biochim. Biophys. Acta 709, 342-352). In order to determine its tissue distribution and molecular forms, a sensitive and specific radioimmunoassay was developed. Gel filtration of rat liver and kidney cytosol demonstrated only one peak of organic anion binder-like immunoreactivity corresponding to a molecular weight of 35 000. Organic anion binder-like immunoreactivity was present in 14 separate tissues. The greatest concentration of the organic anion binder was found in the liver and kidney cytosol, where it constituted 0.5% of the cytoplasmic proteins. Similar hepatic contents were noted for female and male rats. The content of the organic anion binder in the liver and kidney were low in the fetus, but approached adult levels by 2-4 weeks of age. Treatments known to augment hepatic glutathione-S-transferase content had minimal effect on organic anion binder content. In summary, the organic anion binder is a newly recognized cytosolic binding protein, distinct from the glutathione-S-transferases, present in greatest concentration in the liver and kidney. The combination of the protein's binding properties along with its location and ontogeny suggests its possible role in intracellular organic anion transport.
我们最近在大鼠肝脏胞质溶胶中鉴定出一组新的蛋白质，它们具有与谷胱甘肽 - S - 转移酶相当的结合特性，但缺乏转移酶活性。该组分中大部分有机阴离子结合与一种四聚体蛋白质有关，该蛋白质被命名为有机阴离子结合蛋白，分子量为34000（杉山洋、山田隆和卡普洛维茨，N.（1982年）《生物化学与生物物理学报》709，342 - 352）。为了确定其组织分布和分子形式，我们开发了一种灵敏且特异的放射免疫分析法。对大鼠肝脏和肾脏胞质溶胶进行凝胶过滤，结果显示只有一个对应分子量为35000的有机阴离子结合蛋白样免疫反应峰。有机阴离子结合蛋白样免疫反应存在于14个不同的组织中。在肝脏和肾脏胞质溶胶中发现有机阴离子结合蛋白的浓度最高，占细胞质蛋白的0.5%。雌性和雄性大鼠的肝脏含量相似。胎儿肝脏和肾脏中有机阴离子结合蛋白的含量较低，但在2 - 4周龄时接近成年水平。已知能增加肝脏谷胱甘肽 - S - 转移酶含量的处理对有机阴离子结合蛋白含量的影响极小。总之，有机阴离子结合蛋白是一种新发现的胞质结合蛋白，与谷胱甘肽 - S - 转移酶不同，在肝脏和肾脏中浓度最高。该蛋白质的结合特性及其位置和个体发育情况表明它可能在细胞内有机阴离子转运中发挥作用。
