测定催化剂活性在含有固定化酶的渗透膜上的分布。结构问题功能方法的不确定性。
Determination of the distribution of catalyst activity across a permeable membrane containing an immobilized enzyme. Indeterminacy of a functional approach to a structural problem.
作者信息
Bunow B, Caplan S R
出版信息
Biophys J. 1984 Jun;45(6):1065-71. doi: 10.1016/S0006-3495(84)84253-8.
Abstract
Porous membranes were fabricated from collodion and impregnated with papain, inhomogeneously through the thickness of the membrane. These membranes were placed between reservoirs containing N-alpha-benzoyl arginineamide, a substrate for the enzyme papain. The progress of the reaction was monitored by sampling the reservoirs on each side for ammonia, a reaction product. From these data the diffusion coefficient, enzyme activity, and distribution of enzyme activity of the membrane were estimated. The limitations of this approach are discussed in the context of the analysis of biological transport systems.
摘要
用火棉胶制备多孔膜，并使其在膜的整个厚度上不均匀地浸渍木瓜蛋白酶。将这些膜置于含有N-α-苯甲酰精氨酸酰胺（木瓜蛋白酶的一种底物）的储液器之间。通过对两侧储液器中的反应产物氨进行采样来监测反应进程。根据这些数据估算膜的扩散系数、酶活性以及酶活性分布。在生物转运系统分析的背景下讨论了该方法的局限性。
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Determination of the distribution of catalyst activity across a permeable membrane containing an immobilized enzyme. Indeterminacy of a functional approach to a structural problem.测定催化剂活性在含有固定化酶的渗透膜上的分布。结构问题功能方法的不确定性。
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引用本文的文献
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Vectorial product concentration obtained with a permeable immobilized enzyme membrane. A new approach to the analysis of biological transport systems.通过可渗透的固定化酶膜获得的矢量积浓度。生物转运系统分析的一种新方法。
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本文引用的文献
1
Cellular enzymology: the steady-state kinetics of compartmentalized enzymes.
J Theor Biol. 1980 Jun 21;84(4):611-27. doi: 10.1016/s0022-5193(80)80023-3.
2
Structural dynamics of yeast hexokinase during catalysis.酵母己糖激酶催化过程中的结构动力学
Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):43-52. doi: 10.1098/rstb.1981.0058.
3
Papain membrane on a collodion matrix: preparation and enzymic behavior.火棉胶基质上的木瓜蛋白酶膜：制备及酶活性
Science. 1965 Nov 5;150(3697):758-60. doi: 10.1126/science.150.3697.758.
4
Papain-collodion membranes. I. Preparation and properties.木瓜蛋白酶-火棉胶膜。I. 制备与性质
Biochemistry. 1968 Feb;7(2):486-500.
5
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6
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J Cell Biol. 1977 Aug;74(2):605-28. doi: 10.1083/jcb.74.2.605.
7
Chemical reactions and membranes; a macroscopic basis for active transport. II. Non-linear aspects.
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8
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