Rootman J, Ostry A, Gudauskas G
Can J Ophthalmol. 1984 Jun;19(4):187-91.
Two groups of six rabbits each were given 6.25 mg/kg of 6-carbon-14-labelled 5-fluorouracil (5-FU) either subconjunctivally or intravenously. The levels of the drug in the serum, the urine and the ocular humours 0.5, 1, 2, 4, 8 and 12 hours later were measured by high-pressure liquid chromatography, which distinguished between 5-FU and its metabolites. The peak levels of the parent drug in the serum and the urine were similar with the two routes of administration; however, compared with intravenous injection, subconjunctival injection resulted in 125 and 380 times the peak concentration of 5-FU in the anterior chamber aqueous and the vitreous respectively.
将两组各六只兔子分别通过结膜下或静脉内给予6.25毫克/千克的6-碳-14标记的5-氟尿嘧啶（5-FU）。在给药后0.5、1、2、4、8和12小时，通过高压液相色谱法测量血清、尿液和眼房水中药物的水平，该方法可区分5-FU及其代谢产物。两种给药途径下，血清和尿液中母体药物的峰值水平相似；然而，与静脉注射相比，结膜下注射导致前房水和玻璃体中5-FU的峰值浓度分别是其125倍和380倍。
