Inoue F, MacLeod B A, Walker M J
Can J Physiol Pharmacol. 1984 Jun;62(6):658-64. doi: 10.1139/y84-108.
A method of perfusing rat ventricles has been developed which permits the recording of intracellular potentials from up to 10 cell layers below the endocardial surface. Intracellular potentials from all layers remained the same for up to 1.5 h before occlusion. Regional occlusion of flow was produced by occluding the left anterior descending coronary artery and then intracellular potentials were recorded from normal and occluded cells for up to 2 h after occlusion. Immediately after occlusion, marked changes in potentials were recorded from occluded tissue. The greatest change was in the duration of the action potential which showed a marked shortening. There was also a reduction in the maximum rise rate and action potential height with smaller falls in resting membrane potential. Changes were greatest in the deeper cells (layers 6-10) and least in superficial subendocardial cells which were always superfused with perfusion fluid. Occlusion increased the number of quiescent cells (no action potential) as well as the number of cells showing aberrant action potential configurations (e.g., double peaks). Similar changes in intracellular potentials following occlusion were also recorded from the subepicardial surface of anaesthetized rats in situ. Arrhythmias induced by occlusion were similar to those seen in conscious rats following occlusion of a coronary artery.
已开发出一种灌注大鼠心室的方法，该方法能够记录心内膜表面以下多达10个细胞层的细胞内电位。在闭塞前长达1.5小时内，所有层的细胞内电位保持不变。通过闭塞左前降支冠状动脉造成局部血流闭塞，然后在闭塞后长达2小时内记录正常细胞和闭塞细胞的细胞内电位。闭塞后立即记录到闭塞组织的电位发生显著变化。最大的变化在于动作电位的持续时间，其显著缩短。最大上升速率和动作电位高度也降低，静息膜电位下降幅度较小。深层细胞（第6 - 10层）的变化最大，而始终被灌注液超灌注的浅表心内膜下细胞变化最小。闭塞增加了静止细胞（无动作电位）的数量以及显示异常动作电位形态（如双峰）的细胞数量。在麻醉大鼠原位的心外膜表面也记录到闭塞后细胞内电位的类似变化。闭塞诱发的心律失常与清醒大鼠冠状动脉闭塞后所见的心律失常相似。
