Bauermeister A, Sargent J R, Traill K, Nimmo I A
Comp Biochem Physiol B. 1984;78(1):195-8. doi: 10.1016/0305-0491(84)90167-6.
Trout liver cytosol has proteins that bind bile salts and 8-anilino-1-naphthalene sulphonate and that co-elute from Sephadex G-75. The binding of 8-anilino-1-naphthalene sulphonate is decreased by 90% in the presence of 33 microM lithocholate, implying the proteins are the same. Bile salt-binding protein is present in liver, but was not detected in gills, kidney, muscle or intestine. Bile salt-binding protein, partially-purified by Sephadex G-75 chromatography, was resolved into 2 fractions by cholate-affinity chromatography. Fatty acids do not appear to be bound by bile salt-binding protein. No fatty acid-binding protein was detected in trout liver cytosol.
虹鳟鱼肝细胞质中存在能结合胆汁盐和8-苯胺基-1-萘磺酸盐且从葡聚糖G-75共洗脱的蛋白质。在33微摩尔石胆酸盐存在的情况下，8-苯胺基-1-萘磺酸盐的结合减少了90%，这意味着这些蛋白质是相同的。胆汁盐结合蛋白存在于肝脏中，但在鳃、肾脏、肌肉或肠道中未检测到。通过葡聚糖G-75色谱法部分纯化的胆汁盐结合蛋白，经胆酸盐亲和色谱法可分离成2个组分。脂肪酸似乎不与胆汁盐结合蛋白结合。在虹鳟鱼肝细胞质中未检测到脂肪酸结合蛋白。
