Dada M O, Campbell G T, Blake C A
Endocrinology. 1984 Sep;115(3):996-1003. doi: 10.1210/endo-115-3-996.
Female and male rats were injected with monosodium L-glutamate (MSG; 4 mg/g BW) or 0.9% saline as neonates and then decapitated on days 35 and 40 of life, respectively. Trunk blood was collected for RIA of serum GH. Anterior pituitary glands (APGs) were bisected. One half was assayed for GH. The other half was placed in culture medium to study the basal GH release rate. Pituitary sections from additional rats were stained for GH, and morphometric analyses were performed on the GH cells. Treatment with MSG lowered serum GH levels and gland GH content in female but not in male rats. MSG did not alter the gland GH concentration or the basal GH release rate whether expressed per mg APG or per entire gland in either sex. The mean cross-sectional area of GH cells was reduced in either sex of MSG-treated rats. The numerical density of GH cells and the percentage of GH cells in APGs were similar in saline- and MSG-treated rats of either sex. The volume density of GH cells was lower in MSG-treated male rats only. The results suggest that in prepubertal rats which had been given MSG as neonates there is a sex difference in mean serum GH concentration and APG GH content, GH cell size is reduced in both sexes, and the individual GH cells contain normal amounts of GH in spite of their smaller size.
新生雌性和雄性大鼠分别注射L-谷氨酸单钠（MSG；4mg/g体重）或0.9%生理盐水，然后分别在出生后第35天和第40天断头。采集躯干血用于血清生长激素（GH）的放射免疫分析（RIA）。将垂体前叶（APG）一分为二。一半用于检测GH。另一半置于培养基中以研究基础GH释放率。对另外一些大鼠的垂体切片进行GH染色，并对GH细胞进行形态计量分析。MSG处理降低了雌性大鼠的血清GH水平和腺体GH含量，但对雄性大鼠没有影响。无论以每毫克APG还是整个腺体表示，MSG均未改变腺体GH浓度或基础GH释放率。在接受MSG处理的大鼠中，无论雌雄，GH细胞的平均横截面积均减小。在接受生理盐水和MSG处理的大鼠中，无论雌雄，GH细胞的数量密度和GH细胞在APG中的百分比相似。仅在接受MSG处理的雄性大鼠中，GH细胞的体积密度较低。结果表明，在新生期接受MSG处理的青春期前大鼠中，平均血清GH浓度和APG的GH含量存在性别差异，两性的GH细胞大小均减小，尽管单个GH细胞较小，但其GH含量正常。
