Wada Y, Yajima A, Suzuki M, Greenwald G
Endocrinol Jpn. 1984 Apr;31(2):207-15. doi: 10.1507/endocrj1954.31.207.
In order to assess the direct luteotropic effects of steroids, dispersed luteal cells from rats on day 8 or 16 of pregnancy were incubated for 2 h with various concentrations of estradiol (1-1000 ng/ml), testosterone (1-100 ng/ml) or progesterone (1-100 ng/ml). Estradiol, at any concentration, did not cause significant changes in the in vitro accumulation of progesterone, 20 alpha-dihydroprogesterone (20 alpha-OHP) or testosterone by dispersed luteal cells on both days 8 and 16. Addition of testosterone (1-100 ng/ml) increased the accumulation of estradiol on day 8 with estrone synthesized at half to one third the rate of estradiol. On day 16, accumulation of estradiol by dispersed luteal cells was more sensitive to 1 ng/ml testosterone than on day 8 with a concomitant drop in estrone synthesis. Although luteal secretion of estradiol was readily stimulated by testosterone, there were no corresponding changes in the production of progesterone or 20 alpha-OHP. Although the addition of a small amount of progesterone did not cause the anticipated increase in accumulation of 20 alpha-OHP, 100 ng/ml progesterone elicited a slight but significant increase in accumulation of 20 alpha-OHP on day 8. Thus, none of 3 steroids added was found to exert any direct in vitro luteotropic action in the pregnant rat, in acute experiments.
为了评估类固醇的直接促黄体作用，将妊娠第8天或第16天大鼠的分散黄体细胞与不同浓度的雌二醇（1 - 1000 ng/ml）、睾酮（1 - 100 ng/ml）或孕酮（1 - 100 ng/ml）孵育2小时。在第8天和第16天，任何浓度的雌二醇都不会使分散黄体细胞体外孕酮、20α - 二氢孕酮（20α - OHP）或睾酮的积累发生显著变化。添加睾酮（1 - 100 ng/ml）可增加第8天雌二醇的积累，雌酮合成速率为雌二醇的二分之一到三分之一。在第16天，分散黄体细胞对1 ng/ml睾酮的雌二醇积累比第8天更敏感，同时雌酮合成下降。虽然睾酮能轻易刺激黄体分泌雌二醇，但孕酮或20α - OHP的产生没有相应变化。虽然添加少量孕酮并未导致20α - OHP积累预期的增加，但100 ng/ml孕酮在第8天引起20α - OHP积累轻微但显著增加。因此，在急性实验中，未发现添加的3种类固醇在妊娠大鼠中有任何直接的体外促黄体作用。
