Hiraoka Y, Okuda H
Environ Res. 1984 Aug;34(2):262-7. doi: 10.1016/0013-9351(84)90094-x.
To determine how centrum abnormalities in medaka could be used to indicate the degree of water pollution, several rearing experiments were carried out. Medaka eggs were placed at certain points on a stream flowing from an area in which fenitrothion emulsion (organophosphorous pesticide--MEP) was being applied aerially. There was sufficient probability that the eggs were exposed to the MEP. They were brought to the laboratory, and reared in dechlorinated water to adulthood. The external forms and vertebral centra of each adult fish were closely observed. It was found that the incidence of centrum-damaged fish in the exposure experiment groups was significantly higher than in the control and blank experiments, and the influence of the aerial application of MEP was clearly indicated in this manner. Consequently, it was confirmed that centrum abnormalities in medaka may be used as a biological index for indicating the degree of water pollution by agricultural chemicals.
为了确定青鳉的椎体异常如何用于指示水污染程度，进行了几项饲养实验。将青鳉卵放置在一条溪流的特定位置，该溪流源自一个正在空中喷洒杀螟硫磷乳液（有机磷农药——MEP）的区域。鱼卵有足够的概率接触到MEP。将它们带回实验室，在脱氯水中饲养至成年。对每条成年鱼的外形和脊椎椎体进行了仔细观察。结果发现，暴露实验组中椎体受损鱼的发生率显著高于对照组和空白实验，通过这种方式清楚地表明了空中喷洒MEP的影响。因此，证实了青鳉的椎体异常可用作指示农药水污染程度的生物学指标。
