Fu S C, Su S W, Wagner B J, Hart R
Exp Eye Res. 1984 May;38(5):485-95. doi: 10.1016/0014-4835(84)90126-x.
Soluble proteins of individual human normal and nuclear cataractous lenses of 60-70-year-old subjects were collected for this investigation. The average wet weights of both the normal and nuclear cataractous lenses were found to be essentially identical, approximately 230 mg. When the lens proteins, either cortical or nuclear, were subjected to fractionation by Sephadex G-200 sf chromatography, six fractions (F-I to F-VI) were obtained and their respective molecular weights approximated. F-I, which contains alpha-crystallin and high molecular weight aggregates, was subsequently fractionated through a series of Bio-gel A chromatographic columns. The quantities of the proteins and the molecular weights of each fraction were obtained. All the proteins fractionated were subjected to SDS gel electrophoresis by which the molecular weights of the subunits were obtained. The distribution and molecular weights of proteins smaller than 0.2 X 10(6) showed certain changes, more noticeable in the nucleus than in the cortex, between the normal lens and nuclear cataractous lens. For the high-molecular-weight protein aggregates, the major fraction in the lens cortex was found to be in the 5-1.5 X 10(6) range, representing 10 and 12% of the total protein in the normal and cataractous lenses, respectively. The major fraction in the nucleus was found to be greater than 150 X 10(6), representing 11 and 19% for the normal and cataractous lenses respectively. The above data are presented for the first time to show the differences in distribution of the high-molecular-weight proteins in the cortical and nuclear regions, and their respective changes in cataractogenesis. Based upon these data, we are able to calculate the average molecular weights of (1) the soluble cortical and nuclear proteins and (2) the total soluble protein, in the normal and cataractous human lenses.
本研究收集了60至70岁个体的人正常晶状体和核性白内障晶状体的可溶性蛋白质。发现正常晶状体和核性白内障晶状体的平均湿重基本相同，约为230毫克。当晶状体蛋白质（皮质或核质）通过Sephadex G - 200 sf柱色谱进行分级分离时，获得了六个级分（F - I至F - VI），并估算了它们各自的分子量。含有α - 晶状体蛋白和高分子量聚集体的F - I随后通过一系列Bio - gel A色谱柱进行分级分离。获得了每个级分的蛋白质数量和分子量。所有分级分离的蛋白质都进行了SDS凝胶电泳，从而获得了亚基的分子量。在正常晶状体和核性白内障晶状体之间，小于0.2×10⁶的蛋白质的分布和分子量显示出某些变化，在核中比在皮质中更明显。对于高分子量蛋白质聚集体，发现晶状体皮质中的主要级分在5 - 1.5×10⁶范围内，分别占正常晶状体和白内障晶状体总蛋白质的10%和12%。核中的主要级分大于150××10⁶，分别占正常晶状体和白内障晶状体的11%和19%。首次呈现上述数据以显示高分子量蛋白质在皮质和核区域的分布差异及其在白内障形成过程中的各自变化。基于这些数据，我们能够计算出正常和白内障人晶状体中（1）可溶性皮质和核蛋白质以及（2）总可溶性蛋白质的平均分子量。
