胸苷酸合成酶阳性和阴性的小鼠乳腺FM3A癌细胞作为检测胸苷酸合成酶抑制剂的有用系统。
Thymidylate synthetase-positive and -negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors.
作者信息
Balzarini J, de Clercq E, Ayusawa D, Seno T
出版信息
FEBS Lett. 1984 Jul 23;173(1):227-32. doi: 10.1016/0014-5793(84)81052-2.
The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/TS- cells, and their inhibitory effects on FM3A/O cell growth should be readily reversed by exogenous dThd within the concentration range of 5-20 microM.
小鼠乳腺FM3A/O细胞系和胸苷酸（dTMP）合成酶缺陷型FM3A/TS-癌细胞系可被视为一种新型且有用的检测系统，用于检测直接作用于胸苷酸合成酶的核苷类似物。这些化合物应对FM3A/O细胞有抑制作用，而对FM3A/TS-细胞无抑制作用，并且它们对FM3A/O细胞生长的抑制作用应能在5-20微摩尔浓度范围内被外源性脱氧胸苷（dThd）轻易逆转。
Zotero 插件