Karapetyan A T, Minyat E E, Ivanov V I
FEBS Lett. 1984 Jul 23;173(1):243-6. doi: 10.1016/0014-5793(84)81055-8.
An increase in temperature from 20 to 50 degrees C results in the complete transition from the Z to B form of poly[d(G-C)], dissolved in a 55% ethanol-water solution. The transition is fully reversible and displays a slow kinetics. The transition profiles for the free polynucleotide and for that in the presence of ethidium bromide, which is known to stabilize the B form, are obtained by circular dichroism. Based on these data the enthalpy value for the B-Z transition in our conditions is estimated to be delta HBZ = -0.7 kcal/mol.
温度从20摄氏度升高到50摄氏度会导致溶解在55%乙醇 - 水溶液中的聚[d(G - C)]从Z型完全转变为B型。这种转变是完全可逆的，并且呈现出缓慢的动力学过程。通过圆二色性获得了游离多核苷酸以及在已知能稳定B型的溴化乙锭存在下的多核苷酸的转变曲线。基于这些数据，在我们的条件下，B - Z转变的焓值估计为ΔHBZ = -0.7千卡/摩尔。
