Ivkov V G, Pechatnikov V A, Ivkova M N
Gen Physiol Biophys. 1984 Feb;3(1):19-30.
The distribution of amphiphilic and hydrophobic positively charged probes in suspensions of closed membrane vesicles was analysed by the methods of equilibrium thermodynamics. Two versions of the probe location in the membranes are discussed: near the boundary between the polar and hydrocarbon regions, and in the centre of the hydrocarbon region of a lipid bilayer. It is shown that, in the first case, the action of the inside-negative transmembrane potential results in an increased average probe concentration in the lipid phase and in a significant redistribution of the probe in the membrane interior. An inside-positive transmembrane potential causes a decrease in the average probe concentration in membranes, the probe being redistributed in the membrane bilayer. If the probe is sited in the centre of the hydrocarbon layer, the transmembrane potential evokes only an increase or decrease in the probe concentration in membranes at a negative or positive sign of the potential inside the vesicles.
采用平衡热力学方法分析了两亲性和疏水性带正电荷探针在封闭膜泡悬浮液中的分布情况。讨论了探针在膜中的两种定位情况：靠近极性区域与烃类区域的边界处，以及脂质双层烃类区域的中心。结果表明，在第一种情况下，膜内负跨膜电位的作用会导致脂质相中探针平均浓度增加，并使探针在膜内部发生显著重新分布。膜内正跨膜电位会导致膜中探针平均浓度降低，探针在膜双层中重新分布。如果探针位于烃层中心，跨膜电位仅在囊泡内部电位为负或正时引起膜中探针浓度的增加或降低。
