16,16-二甲基前列腺素E2对大鼠α-萘基异硫氰酸酯诱导的实验性胆管炎的保护作用。

16,16-dimethyl-PGE2 protection against alpha-naphthylisothiocyanate-induced experimental cholangitis in the rat.

作者信息

Ruwart M J, Rush B D, Friedle N M, Stachura J, Tarnawski A

出版信息

Hepatology. 1984 Jul-Aug;4(4):658-60. doi: 10.1002/hep.1840040415.

Abstract

Male rats were treated with subcutaneous vehicle or 16,16-dimethyl-PGE2 (dmPGE2, 100 micrograms per kg), 24, 18 and 0.5 hr prior to and 6, 24 and 30 hr after challenge with oral alpha-naphthylisothiocyanate (ANIT, 30 mg per kg). Forty-eight hours after challenge, rats were sacrificed by decapitation; serum and liver samples were taken for biochemical and histological analysis, respectively. Rats treated with vehicle (2% ethanol in saline) and ANIT exhibited elevations in alkaline phosphatase, SGPT and bilirubin as well as cholangitis and mild parenchymal necrosis. Rats treated with dmPGE2 and ANIT had normal serum biochemical findings, no necrosis and only mild proliferation of bile duct epithelium. Thus, dmPGE2 may be able to protect the rat liver against the deleterious effects of orally administered ANIT.

摘要

在给雄性大鼠口服α-萘基异硫氰酸酯（ANIT，每千克30毫克）进行激发前24、18和0.5小时以及激发后6、24和30小时，分别皮下注射赋形剂或16,16-二甲基前列腺素E2（dmPGE2，每千克100微克）。激发后48小时，通过断头处死大鼠；分别采集血清和肝脏样本进行生化和组织学分析。用赋形剂（盐水中含2%乙醇）和ANIT处理的大鼠碱性磷酸酶、谷丙转氨酶和胆红素升高，同时出现胆管炎和轻度实质坏死。用dmPGE2和ANIT处理的大鼠血清生化指标正常，无坏死，仅胆管上皮有轻度增生。因此，dmPGE2可能能够保护大鼠肝脏免受口服ANIT的有害影响。