从多株粪肠球菌中分离并鉴定可溶性肽聚糖。
Isolation and characterization of soluble peptidoglycan from several strains of Streptococcus faecium.
作者信息
Barrett J F, Shockman G D
出版信息
J Bacteriol. 1984 Aug;159(2):511-9. doi: 10.1128/jb.159.2.511-519.1984.
Abstract
Two phenotypically autolysis-deficient strains of Streptococcus faecium ATCC 9790 were shown to produce high-molecular-weight, soluble, linear, uncross-linked peptidoglycan when incubated with benzylpenicillin in a wall medium which permits cell wall synthesis (wall thickening) but not balanced growth. This high-molecular-weight s-peptidoglycan was shown to have a molecular weight of 46,000 to 54,000, lack peptide cross-links, and be virtually devoid of accessory wall polymers. It was hydrolyzed by hen egg white lysozyme and the endogenous, autolytic N-acetylmuramidase of S. faecium, but was not attacked by proteinases. Chemical analyses of the polymer are consistent with the following structure, where n is the number of repeating disaccharide units: (formula; see text).
摘要
已表明，粪肠球菌ATCC 9790的两株表型自溶缺陷菌株，在允许细胞壁合成（细胞壁增厚）但不允许平衡生长的壁培养基中与苄青霉素一起孵育时，会产生高分子量、可溶、线性、未交联的肽聚糖。这种高分子量的s-肽聚糖的分子量为46,000至54,000，缺乏肽交联，并且几乎不含辅助壁聚合物。它可被鸡蛋清溶菌酶和粪肠球菌的内源性自溶N-乙酰胞壁酸酶水解，但不被蛋白酶攻击。该聚合物的化学分析与以下结构一致，其中n是重复二糖单元的数量：（分子式；见正文）。
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