Wall L A, Byers D M, Meighen E A
J Bacteriol. 1984 Aug;159(2):720-4. doi: 10.1128/jb.159.2.720-724.1984.
Incubation of soluble extracts from Vibrio harveyi with [3H]tetradecanoic acid (+ ATP) resulted in the acylation of several polypeptides, including proteins with molecular masses near 20 kilodaltons (kDa), and at least five polypeptides in the 30- to 60-kDa range. However, in growing cells pulse-labeled in vivo with [3H]tetradecanoic acid, only three of these polypeptides, with apparent molecular masses of 54, 42, and 32 kDa, were specifically labeled. When extracts were acylated with [3H] tetradecanoyl coenzyme A, on the other hand, only the 32-kDa polypeptide was labeled. When luciferase-containing dark mutants of V. harveyi were investigated, acylated 32-kDa polypeptide was not detected in a fatty acid-stimulated mutant, whereas the 42-kDa polypeptide appeared to be lacking in a mutant defective in aldehyde synthesis. Acylation of both of these polypeptides also increased specifically during induction of bioluminescence in V. harveyi. These results suggest that the role of the 32-kDa polypeptide is to supply free fatty acids, whereas the 42-kDa protein may be responsible for activation of fatty acids for their subsequent reduction to form the aldehyde substrates of the bioluminescent reaction.
用[3H]十四烷酸（+ATP）孵育哈维氏弧菌的可溶性提取物，导致几种多肽发生酰化，包括分子量接近20千道尔顿（kDa）的蛋白质，以及30至60 kDa范围内的至少五种多肽。然而，在用[3H]十四烷酸进行体内脉冲标记的生长细胞中，这些多肽中只有三种，表观分子量分别为54、42和32 kDa，被特异性标记。另一方面，当提取物用[3H]十四烷酰辅酶A进行酰化时，只有32 kDa的多肽被标记。当研究含有荧光素酶的哈维氏弧菌黑暗突变体时，在脂肪酸刺激的突变体中未检测到酰化的32 kDa多肽，而在醛合成缺陷的突变体中似乎缺乏42 kDa的多肽。在哈维氏弧菌生物发光诱导过程中，这两种多肽的酰化也都特异性增加。这些结果表明，32 kDa多肽的作用是提供游离脂肪酸，而42 kDa蛋白质可能负责脂肪酸的活化，以便随后将其还原形成生物发光反应的醛底物。
