雌激素受体与雌激素受体结合因子的结合和解离受镁离子（Mg2+）调控。

Association and dissociation of estrogen receptor with estrogen receptor-binding factors is regulated by Mg2+.

作者信息

Fukai F, Murayama A

出版信息

J Biochem. 1984 Apr;95(4):1227-30. doi: 10.1093/oxfordjournals.jbchem.a134715.

DOI:10.1093/oxfordjournals.jbchem.a134715

Abstract

It was recently shown that uterine estrogen receptor (ER) is translocated from the cytoplasm into the nucleus in the form of vero-ER X E (basic ER molecule bound with estradiol), and the translocation is inhibited by the specific binding of vero-ER X E with the cytoplasmic protein factors designated as ER-binding factors (ERBFs) ["5S" ER-forming factor ("5S" ER-FF), "6S" ER-FF, "8S" ER-FF] [Murayama, A. & Fukai, F. (1983) J. Biochem. 94, 511]. It was found that the specific interaction of vero-ER X E with the ERBFs is regulated by Mg2+ at physiological concentrations. The apparent Kd values of vero-ER X E for the ERBFs ["5S" ER-FF, 2.7 X 10(-9) M; "6S" ER-FF, 6.0 X 10(-8) M; "8S" ER-FF, 3.5 X 10(-8) M] observed in the presence of 1 mM Mg2+ were all increased over 10 times as compared with in the absence of Mg2+. The inhibitory effects of the ERBFs on the nuclear translocation of vero-ER X E were reduced by Mg2+.

摘要

最近有研究表明，子宫雌激素受体（ER）以vero-ER X E（与雌二醇结合的碱性ER分子）的形式从细胞质转运至细胞核，并且这种转运受到vero-ER X E与被称为ER结合因子（ERBFs）的细胞质蛋白因子[“5S”ER形成因子（“5S”ER-FF）、“6S”ER-FF、“8S”ER-FF]特异性结合的抑制[村山，A. & 深井，F.（1983年）《生物化学杂志》94卷，511页]。研究发现，vero-ER X E与ERBFs的特异性相互作用受生理浓度的Mg2+调控。在存在1 mM Mg2+的情况下观察到的vero-ER X E对ERBFs[“5S”ER-FF，2.7×10−9 M；“6S”ER-FF，6.0×10−8 M；“8S”ER-FF，3.5×10−8 M]的表观解离常数（Kd）值与不存在Mg2+时相比均增加了10倍以上。Mg2+可降低ERBFs对vero-ER X E核转运的抑制作用。