Schneider D J, Roe A L, Mayer R J, Que L
J Biol Chem. 1984 Aug 10;259(15):9699-703.
The 2,3-dihydroxybenzoate and thioglycolate complexes of iron(III)-ovotransferrin have been studied with resonance Raman and extended x-ray absorption fine structure spectroscopies, respectively, to obtain evidence for the coordination of the synergistic anion to the iron center. The dihydroxybenzoate complex exhibits resonance-enhanced Raman vibrations arising from both the endogenous tyrosinates and the added dihydroxybenzoate. A comparison of the extended x-ray absorption fine structure spectra of the carbonate and thioglycolate complexes shows a large feature at about 1.95 A assigned to Fe-(O,N) interactions. The latter complex exhibits an added feature at 2.32 A assigned to an Fe-S interaction. These experiments demonstrate that the Lewis base functions in the synergistic anions coordinate to the iron in ovotransferrin.
分别利用共振拉曼光谱和扩展X射线吸收精细结构光谱对铁（III）-卵转铁蛋白的2,3-二羟基苯甲酸盐和巯基乙酸盐配合物进行了研究，以获取协同阴离子与铁中心配位的证据。二羟基苯甲酸盐配合物表现出源于内源性酪氨酸盐和添加的二羟基苯甲酸盐的共振增强拉曼振动。碳酸盐和巯基乙酸盐配合物的扩展X射线吸收精细结构光谱比较显示，在约1.95 Å处有一个大的特征峰，归因于Fe-(O,N)相互作用。后一种配合物在2.32 Å处表现出一个归因于Fe-S相互作用的附加特征峰。这些实验表明，协同阴离子中的路易斯碱官能团与卵转铁蛋白中的铁配位。
