Weiss R L
J Cell Sci. 1984 Apr;67:133-43. doi: 10.1242/jcs.67.1.133.
The cytoskeleton of Chlamydomonas reinhardtii gametes has been studied by electron microscopy. The microtubular system, consisting of four flagellar roots inserted into the basal apparatus, is shown to include two daughter basal bodies and two striated fibres, newly described in this report. One new fibre associates with the 3-over-1 root and is similar to its counterpart, the striated fibre of the 2-member root. These similar root fibres connect each daughter basal body to the V-shaped microtubular root pair. The other new striated fibre joins the daughter basal body to both flagellar roots and is similar to the proximal striated fibre. In mt+ gametes, the conventional root microtubules make direct contact with the doublet zone of the non-activated mating structure. During activation, doublet zone microfilaments associate with the daughter basal body and the finely striated fibre of the 3-over-1 root. These observations suggest that the cytoskeleton acts as a scaffolding for membrane extension by the mt+ mating structure microfilaments.
通过电子显微镜对莱茵衣藻配子的细胞骨架进行了研究。微管系统由插入基部装置的四条鞭毛根组成，研究表明该系统包括两个子基体和两条横纹纤维，这是本报告中首次描述的。一条新纤维与3上1根相关联，与其对应物（2成员根的横纹纤维）相似。这些相似的根纤维将每个子基体连接到V形微管根对。另一条新的横纹纤维将子基体与两条鞭毛根相连，与近端横纹纤维相似。在mt +配子中，传统的根微管与未激活的交配结构的双联体区域直接接触。在激活过程中，双联体区域微丝与子基体和3上1根的细横纹纤维相关联。这些观察结果表明，细胞骨架作为mt +交配结构微丝进行膜延伸的支架。
