Skinner S J, Grego B, Hearn M T, Liggins G C
J Chromatogr. 1984 Jun 8;308:111-9. doi: 10.1016/s0021-9673(01)87538-5.
Procedures for the separation of alpha 1(I), alpha 2(I), alpha 1(II) and alpha 1 (III) chains of human collagen by reversed-phase high-performance liquid chromatography are described. The influence of several different chromatographic parameters (stationary phase, mobile phase and temperature) has been examined and procedures to optimise resolution presented. These reversed-phase high-performance liquid chromatographic conditions also permit the separation of collagen alpha 1(I), alpha 2(I), alpha 1(II) and alpha 1(III) monomers from their corresponding dimeric beta- and gamma-components.
本文描述了通过反相高效液相色谱法分离人胶原蛋白α1(I)、α2(I)、α1(II)和α1(III)链的方法。研究了几种不同色谱参数（固定相、流动相和温度）的影响，并提出了优化分离度的方法。这些反相高效液相色谱条件还能将胶原蛋白α1(I)、α2(I)、α1(II)和α1(III)单体与其相应的二聚体β-和γ-组分分离。
