酶联交叉免疫电泳：一种检测人类血吸虫病中过敏原及其相应抗体的技术。

Enzyme-linked crossed immunoelectrophoresis: a technique for detection of allergens and their respective antibodies in human schistosomiasis.

作者信息

Reiner G, Pauly H E, Zahner H

出版信息

J Immunol Methods. 1984 Aug 3;72(1):289-95. doi: 10.1016/0022-1759(84)90457-5.

Abstract

A method is described which allows the demonstration of allergens in complex antigens and their respective antibodies. Schistosoma mansoni antigens are separated in agarose by 2-dimensional electrophoresis, using an anti-S. mansoni serum from goats in the second dimension. After extensive washing test serum is spread over the gel and allowed to bind to the precipitated antigens. After further extensive washing, horseradish peroxidase-coupled anti-IgE antibodies are put on the plate and allowed to react. Bound antiserum is visualized with tetramethylbenzidine as a substrate. In pooled sera from schistosomiasis patients at least 7 antigen fractions of adult S. mansoni and 2 of cercarial antigen reacted with IgE antibodies. No reaction was found in normal sera.

摘要

本文描述了一种可用于展示复杂抗原及其相应抗体中过敏原的方法。曼氏血吸虫抗原通过二维电泳在琼脂糖中进行分离，在第二维电泳中使用山羊抗曼氏血吸虫血清。经过大量洗涤后，将测试血清铺展在凝胶上，并使其与沉淀的抗原结合。进一步大量洗涤后，将辣根过氧化物酶偶联的抗IgE抗体置于平板上并使其反应。用四甲基联苯胺作为底物使结合的抗血清显色。在血吸虫病患者的混合血清中，至少7种曼氏血吸虫成虫抗原组分和2种尾蚴抗原与IgE抗体发生反应。在正常血清中未发现反应。