Uilenbroek J T, van der Linden R
J Endocrinol. 1984 Aug;102(2):245-50. doi: 10.1677/joe.0.1020245.
The effect of prolactin on follicular oestradiol production was studied in rat ovaries in which luteal tissue was absent. A silicone tube containing progesterone was implanted before first ovulation and removed 14 days later. This resulted in the presence of preovulatory follicles 2 days later and ovulation 60 h after removal of the implant. Prolactin concentrations were raised either by injection of purified prolactin or by implantation of pituitary tissue under the kidney capsule. Injections with 100 or 200 micrograms prolactin starting at the time of removal of the implant (16.00 h on day 0) had no effect on in-vitro oestradiol production by preovulatory follicles obtained on day 2 (day of pro-oestrus). However, implantation of two pituitary glands under the kidney capsule, 2 weeks before progesterone removal, resulted in significantly lower follicular oestradiol production, although ovulation was not inhibited. Lowering of serum prolactin by injections of bromocriptine resulted in an increased follicular oestradiol production. These results indicate that, in addition to its well-known luteotrophic effect, prolactin can have a direct inhibitory effect on follicular oestradiol production. This effect might contribute to the reduced fertility seen during hyperprolactinaemia.
在无黄体组织的大鼠卵巢中研究了催乳素对卵泡雌二醇生成的影响。在首次排卵前植入一根含孕酮的硅胶管，并在14天后取出。这导致2天后出现排卵前卵泡，并在取出植入物后60小时排卵。通过注射纯化的催乳素或在肾包膜下植入垂体组织来提高催乳素浓度。从取出植入物时（第0天16:00）开始注射100或200微克催乳素，对第2天（动情前期）获得的排卵前卵泡的体外雌二醇生成没有影响。然而，在取出孕酮前2周在肾包膜下植入两个垂体，尽管排卵未受抑制，但卵泡雌二醇生成显著降低。注射溴隐亭降低血清催乳素水平导致卵泡雌二醇生成增加。这些结果表明，除了其众所周知的促黄体作用外，催乳素对卵泡雌二醇生成可具有直接抑制作用。这种作用可能是高催乳素血症期间生育力降低的原因之一。
