Ecob M
J Neurol Sci. 1984 May;64(2):175-82. doi: 10.1016/0022-510x(84)90035-2.
The location of neuromuscular junctions which form in vitro between regenerating adult mouse muscle fibres and sections of embryonic mouse spinal cord was examined. The position of the original motor end-plates on the explanted muscle fibres was determined by using either rhodamine-labelled alpha-bungarotoxin (R alpha BT) binding to the acetylcholine receptors, or by stains to demonstrate acetylcholinesterase (AChE) also located at the end-plate. In this culture system, the explanted muscle fibres degenerate and regenerate to form new myotubes which develop cross-striations and contractions. The location of the newly-formed neuromuscular junctions in these mature cultures was then demonstrated using R alpha BT-binding to acetylcholine receptors, silver impregnation and cholinesterase techniques. Less than half the new neuromuscular junctions were at the original end-plate areas indicating that, at least in this system, junctions can form at sites other than those of the original end-plate.
对成年小鼠再生肌纤维与胚胎小鼠脊髓切片在体外形成的神经肌肉接头的位置进行了检查。通过使用与乙酰胆碱受体结合的罗丹明标记的α-银环蛇毒素（RαBT），或通过染色以显示同样位于终板处的乙酰胆碱酯酶（AChE），来确定移植的肌纤维上原始运动终板的位置。在这个培养系统中，移植的肌纤维退化并再生，形成新的肌管，这些肌管会出现横纹并产生收缩。然后，使用与乙酰胆碱受体结合的RαBT、银浸染和胆碱酯酶技术，在这些成熟培养物中显示新形成的神经肌肉接头的位置。不到一半的新神经肌肉接头位于原始终板区域，这表明，至少在这个系统中，接头可以在原始终板以外的部位形成。
