Paul D H, Roberts B L
J Physiol. 1984 Jul;352:1-16. doi: 10.1113/jphysiol.1984.sp015274.
Patterns of activity of cerebellar neurones in response to cutaneous stimulation and during spontaneous, swimming-like movements were examined, using microelectrodes, in decerebrate dogfish (Scyliorhinus canicula). Continuous swimming movements, lasting for several hours, were obtained in fish in which the medial longitudinal fasciculus (m.l.f.) was lesioned in the rhombencephalon. Purkinje cells (P cells) and some stellate cells (S cells) were observed to discharge rhythmically, in phase with swimming movements. These units were distributed throughout the cerebellum, but with no apparent somatotopic distribution. After curarization, rhythmic motor discharges could still be recorded from ventral roots and phase locked P cell discharges were recorded from the cerebellum. P cells that discharged rhythmically during active swimming movements, did not do so when the body was oscillated passively during quiescent periods. Cutaneous stimulation evoked burst discharges in many P cells at long latency (ca. 100 ms) both before and after curarization and whether or not a rhythmic motor output was being generated. In rhythmically discharging units, a similar response was obtained when cutaneous stimulation was applied during that part of a cycle when the unit was most or least active. It was concluded that cerebellar neurones discharged in phase with the output of the spinal locomotory rhythm generators and independently of peripheral sensory feed-back.
运用微电极，在去脑的角鲨（Scyliorhinus canicula）中检测了小脑神经元对皮肤刺激的反应模式以及在自发的类似游泳运动期间的活动模式。在内侧纵束（m.l.f.）在菱脑受损的鱼类中，获得了持续数小时的连续游泳运动。观察到浦肯野细胞（P细胞）和一些星状细胞（S细胞）与游泳运动同步有节奏地放电。这些神经元分布于整个小脑，但没有明显的躯体定位分布。箭毒化后，仍可从腹根记录到有节奏的运动放电，并且从小脑记录到与P细胞放电锁相的放电。在主动游泳运动期间有节奏地放电的P细胞，在静止期身体被动摆动时则不如此放电。皮肤刺激在箭毒化前后以及无论是否产生有节奏的运动输出时，均在许多P细胞中诱发长潜伏期（约100毫秒）的爆发性放电。在有节奏放电的神经元中，当在一个周期中该神经元最活跃或最不活跃的部分施加皮肤刺激时，可获得类似的反应。得出的结论是，小脑神经元与脊髓运动节律发生器的输出同步放电，且与外周感觉反馈无关。
