Watanabe M, Tsuru S, Aiso S, Hibi T, Yoshida T, Asakura H, Zinnaka Y, Tsuchiya M
J Clin Lab Immunol. 1984 May;14(1):29-34.
Lymphocyte proliferative response to phytohemagglutinin (PHA) and relevance of serum factors to the response were studied in 12 patients with Crohn's disease (CD). The lymphocyte proliferative response was markedly reduced in patients with Crohn's disease (S.I. = 38.8 +/- 36.8) (mean +/- SD), as compared with normal controls (S.I. = 100.6 +/- 28.6) (p less than 0.01). In addition, the effect of sera from patients with CD or 10 patients with ulcerative colitis (UC) who had extremely impaired lymphocyte responsiveness to PHA on the proliferative response of normal lymphocytes to PHA was also measured. Sera from CD patients had a marked suppressive effect on the blastogenesis of normal lymphocytes (S.I. = 46.4 +/- 28.5), as compared with normal sera (S.I. = 126.2 +/- 14.7) (p less than 0.001). On the other hand, UC sera did not suppress the blastogenesis of normal lymphocytes (S.I. = 114.9 +/- 27.7). Moreover, serum immunosuppressive acidic protein (IAP) levels in patients' sera were measured by single radial immunodiffusion assay. A marked increase in serum IAP levels was revealed both in CD patients (780 +/- 470 micrograms/ml) and in UC patients (601 +/- 278 micrograms/ml), as compared with normal controls (376 +/- 92 micrograms/ml) (p less than 0.001). But there was no precise correlation between the suppressive effect of sera and serum IAP levels in patients with CD. Thus, we demonstrated an impairment of the lymphocyte responsiveness to PHA in CD patients and the possible existence of immunosuppressive factors which is not identical with IAP in the sera from patients with CD.
对12例克罗恩病（CD）患者的淋巴细胞对植物血凝素（PHA）的增殖反应以及血清因子与该反应的相关性进行了研究。与正常对照组（刺激指数=100.6±28.6）相比，克罗恩病患者的淋巴细胞增殖反应显著降低（刺激指数=38.8±36.8）（平均值±标准差）（p<0.01）。此外，还检测了10例淋巴细胞对PHA反应极差的溃疡性结肠炎（UC）患者或CD患者的血清对正常淋巴细胞对PHA增殖反应的影响。与正常血清（刺激指数=126.2±14.7）相比，CD患者的血清对正常淋巴细胞的母细胞形成有显著抑制作用（刺激指数=46.4±28.5）（p<0.001）。另一方面，UC患者的血清并未抑制正常淋巴细胞的母细胞形成（刺激指数=114.9±27.7）。此外，通过单向放射免疫扩散试验测定了患者血清中的免疫抑制酸性蛋白（IAP）水平。与正常对照组（376±92微克/毫升）相比，CD患者（780±470微克/毫升）和UC患者（601±278微克/毫升）的血清IAP水平均显著升高（p<0.001）。但CD患者血清的抑制作用与血清IAP水平之间没有精确的相关性。因此，我们证明了CD患者淋巴细胞对PHA的反应受损，并且CD患者血清中可能存在与IAP不同的免疫抑制因子。
