Hoss W, Formaniak M
Membr Biochem. 1984;5(3):209-23. doi: 10.3109/09687688409150279.
Chlorotetracycline (CT) was used as a fluorescent probe for membrane calcium with intact synaptosomes. The net increase in fluorescence intensity at 520 nm, which is a measure of membrane-bound Ca, increases with increasing Ca, saturating in the millimolar range. Membrane Ca can also be detected in the absence of added external Ca. Potassium-induced depolarization of synaptosomes leads to an increase in membrane Ca, reaching a new steady-state value within 5 min. Neither opiates nor phenytoin affected synaptosomal membrane Ca. Relatively high concentrations of chlorotetracycline increased depolarization-induced uptake of 45Ca into synaptosomes. The data suggest that the Ca-CT complex binds to synaptic plasma membranes, and that depolarization-induced Ca influx results in increased Ca binding to the internal surface of the plasma membrane and/or other internal membranes.
金霉素（CT）被用作完整突触体膜钙的荧光探针。520nm处荧光强度的净增加是膜结合钙的一种度量，它随钙浓度增加而增加，在毫摩尔范围内达到饱和。在未添加外部钙的情况下也能检测到膜钙。钾诱导的突触体去极化导致膜钙增加，在5分钟内达到新的稳态值。阿片类药物和苯妥英均不影响突触体膜钙。相对高浓度的金霉素增加了去极化诱导的45Ca进入突触体的摄取。数据表明，钙-金霉素复合物与突触质膜结合，去极化诱导的钙内流导致钙与质膜内表面和/或其他内膜的结合增加。
