一种用于检测细胞培养上清液中IgG的定量酶联免疫吸附测定法。
A quantitative ELISA for IgG in cell culture supernatants.
作者信息
Wakefield E F, Shelton M J, Hosking C S
出版信息
Clin Chim Acta. 1982 Aug 18;123(3):303-10. doi: 10.1016/0009-8981(82)90175-9.
The measurement of IgG, using a sandwich-type enzyme-linked immunosorbent assay (ELISA), is described. The assay is specific, sensitive, and simple to perform. Purification of the antibody, prior to attachment to the plastic solid phase, results in a more sensitive test. Optimum standardisation of the reagents and conditions is described and discussed. The assay is capable of detecting IgG to 2.5 X 10(-5) IU/ml (approximately equal to 3 ng/ml) and is useful for measuring IgG produced by cells in culture. The coefficient of variation within assays is 6.0% and between assays is 7.7%.
本文描述了使用夹心型酶联免疫吸附测定法（ELISA）测量IgG的方法。该测定法具有特异性、敏感性，且操作简单。在将抗体附着到塑料固相之前进行纯化，可使检测更加灵敏。文中描述并讨论了试剂和条件的最佳标准化方法。该测定法能够检测低至2.5×10⁻⁵IU/ml（约等于3 ng/ml）的IgG，可用于测量培养细胞产生的IgG。测定法内的变异系数为6.0%，不同测定法之间的变异系数为7.7%。
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