Du Pasquier L, Horton J D
Eur J Immunol. 1982 Jul;12(7):546-51. doi: 10.1002/eji.1830120704.
The restorative potential of isogeneic and fully allogeneic thymic implants to restore in vivo antibody responsiveness to a thymus-dependent antigen has been examined in thymectomized clawed toads. Isogeneic clones (Xenopus laevis/gilli and X. laevis/muelleri hybrids) of known major histocompatability complex (MHC) haplotypes were thymectomized at 5-7 days, implanted with MHC-identical or -mismatched (normal or irradiated ) thymuses from 5-week-old larvae and 4 months later injected with dinitrophenylated keyhole limpet hemocyanin. IgM antibody responsiveness (measured by phage inactivation) was restored in terms of its quantity, affinity and specificity in those thymectomized animals given two haplotype-different thymuses. Isoelectric focusing data revealed that low molecular weight antibody production was also restored with isogeneic and allogeneic thymus and that the antibody spectrotypes were of the host type. The finding that in vivo T-B collaboration in thymectomized animals develops normally following thymus implantation, even when the thymus genotype is disparate from the host, is discussed both in the light of previous experiments revealing MHC restriction of Xenopus T helper cells in vitro and in terms of the role of the thymus in promoting adaptive differentiation of this lymphocyte subset.
在胸腺切除的爪蟾中，研究了同基因和完全异基因胸腺植入物恢复体内对胸腺依赖性抗原抗体反应性的修复潜力。已知主要组织相容性复合体（MHC）单倍型的同基因克隆（非洲爪蟾/吉利爪蟾和非洲爪蟾/米勒爪蟾杂种）在5 - 7日龄时进行胸腺切除，植入来自5周龄幼虫的MHC相同或不匹配（正常或辐照）的胸腺，4个月后注射二硝基苯基化的钥孔戚血蓝蛋白。在给予两个单倍型不同胸腺的胸腺切除动物中，IgM抗体反应性（通过噬菌体失活测量）在数量、亲和力和特异性方面均得以恢复。等电聚焦数据显示，同基因和异基因胸腺植入后，低分子量抗体的产生也得以恢复，且抗体光谱类型为宿主型。鉴于先前实验揭示非洲爪蟾T辅助细胞在体外的MHC限制以及胸腺在促进该淋巴细胞亚群适应性分化中的作用，讨论了胸腺切除动物在胸腺植入后体内T - B协作正常发展这一发现，即便胸腺基因型与宿主不同。
