Kulesz-Martin M F, Birnkrant A, McLimans W F
Exp Cell Biol. 1982;50(4):207-15.
We have reported that human skin, cultured in a controlled environment system (steady state), yields epidermis-like growth from adult donor split thickness specimens. A procedure for transplantation of such cellular outgrowths to athymic nude mice for functional and morphological evaluations is reported. Successful transplants were definitely scored by the presence of histologic epidermal markers and human glucose phosphate isomerase. We suggest that the transplantation procedures reported here may contribute to the knowledge required for the use of autologous cultured skin in patients with large skin wounds.
我们已经报道过，在受控环境系统（稳态）中培养的人皮肤，能从成年供体的中厚皮片标本中产生类似表皮的生长物。本文报道了一种将这种细胞生长物移植到无胸腺裸鼠体内进行功能和形态学评估的方法。通过组织学表皮标记物和人葡萄糖磷酸异构酶的存在明确判定移植成功。我们认为，这里报道的移植程序可能有助于获取在大面积皮肤伤口患者中使用自体培养皮肤所需的知识。
