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利用温度敏感型（ts）和冷敏感型（cs）突变体确定酵母核分裂途径中基因功能的顺序

Determination of the order of gene function in the yeast nuclear division pathway using cs and ts mutants.

作者信息

Moir D, Botstein D

出版信息

Genetics. 1982 Apr;100(4):565-77. doi: 10.1093/genetics/100.4.565.

DOI:10.1093/genetics/100.4.565
PMID:6749599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1201832/
Abstract

Cold-sensitive (cs) and heat-sensitive (ts) conditional-lethal mutations that affect specifically the cell division cycle of budding yeast (Saccharomyces cerevisiae) were used to determine the order of gene function. Reciprocal temperature-shift experiments using cs-ts double mutants revealed a detailed order of function among genes whose execution points and mutant phenotypes are very similar. The data suggest that the nuclear branch of the overall cell-cycle pathway itself contains at least one branch.

摘要

利用影响芽殖酵母（酿酒酵母）细胞分裂周期的冷敏感（cs）和热敏感（ts）条件致死突变来确定基因功能的顺序。使用cs-ts双突变体进行的双向温度转换实验揭示了执行点和突变表型非常相似的基因之间详细的功能顺序。数据表明，整个细胞周期途径的核分支本身至少包含一个分支。

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本文引用的文献

1
Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor.通过一种可扩散的性因子使单倍体酵母细胞在DNA合成起始阶段发生可逆性停滞。
Exp Cell Res. 1973 Jan;76(1):99-110. doi: 10.1016/0014-4827(73)90424-2.