De Simone C, Donelli G, Meli D, Molinari A, Rosati F, Sorice F
Immunobiology. 1982;162(2):116-27. doi: 10.1016/S0171-2985(11)80023-9.
Receptors for IgM were detected on peripheral blood human eosinophils by a rosette technique with ox red blood cells coated with the IgM fraction of the specific immunserum. Between 14% and 43% (mean 27%) FcmuR positive cells were found after an overnight incubation period at 37 degrees C by using this technique. The specificity of the receptors for IgM was assessed by studying the inhibitory capacity of purified human IgM in the rosette assay. From an ultrastructural point of view, the EAM rosette-forming cells are mature eosinophilic granulocytes characterized by a nucleus with a variable number of lobes and a certain number of "first type" granules partially or totally devoid of their content.
采用以特异性免疫血清的IgM部分包被的牛红细胞通过玫瑰花结技术在外周血人嗜酸性粒细胞上检测到了IgM受体。使用该技术在37℃过夜孵育后，发现14%至43%（平均27%）的FcmuR阳性细胞。通过研究纯化的人IgM在玫瑰花结试验中的抑制能力来评估IgM受体的特异性。从超微结构角度来看，形成EAM玫瑰花结的细胞是成熟的嗜酸性粒细胞，其特征为细胞核有不同数量的叶以及一定数量的“第一型”颗粒，这些颗粒部分或完全没有其内容物。
