Bartsch M, Rienhardt P, Subramanian A R
J Biol Chem. 1982 Oct 25;257(20):12060-2.
A reinvestigation of the copy number of protein S6 in Escherichia coli ribosomes shows that there is only one copy of S6 in 30 S subunits and in 70 S ribosomes. The previously reported higher value (Subramanian, A. R. (1980) J. Biol. Chem. 255, 6941-6946) is shown to arise from the presence in the usual ribosome preparations of a protein which co-electrophoreses with S6 but does not react with S6 antiserum. This protein is removed when ribosomes are purified by passage through a sucrose gradient.
对大肠杆菌核糖体中蛋白质S6拷贝数的重新研究表明，在30 S亚基和70 S核糖体中S6仅有一个拷贝。先前报道的较高数值（Subramanian, A. R. (1980) J. Biol. Chem. 255, 6941 - 6946）被证明是由于在常规核糖体制剂中存在一种与S6共电泳但不与S6抗血清反应的蛋白质。当核糖体通过蔗糖梯度纯化时，这种蛋白质会被去除。
