White L A, Chappell W A
J Clin Microbiol. 1982 Aug;16(2):253-6. doi: 10.1128/jcm.16.2.253-256.1982.
Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing greater than or equal to 10(9) 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% beta-propiolactone or by heating at 56 degrees for greater than or equal to 30 min. Viable virus in tissue impression smears was inactivated by acetone fixation at 50 degrees C for greater than or equal to 30 min or by immersion in 0.1% beta-propiolactone at 37 degrees C for 2 h. Inactivated reagents gave specific and sensitive reactions in the fluorescent rabies antibody test.
本文描述了用于荧光狂犬病抗体检测的试剂中狂犬病病毒的灭活程序。每毫升含大于或等于10(9)个50%致死剂量病毒的小鼠脑吸附悬液，通过用0.1%β-丙内酯处理或在56℃加热大于或等于30分钟使其失去感染性。组织印片涂片中的活病毒通过在50℃丙酮固定大于或等于30分钟或在37℃浸入0.1%β-丙内酯中2小时而被灭活。灭活后的试剂在荧光狂犬病抗体检测中产生特异性和灵敏的反应。
